Identification of abiotic stress microRNAs in Avicennia marina

60 days old Avicennia marina plants were subjected to 500mM salt and 15% PEG induced drought stress for 48h. 3 barcoded small RNASeq libraries were produced from leaf and root tissues, one from each treatment (control, drought and salt stressed). The leaf and root samples were pooled in equal concentration to reduce the number of libraries. All libraries were sequenced using Illumina NextSeq500 platform to generate 75bp single end reads. Quality of reads was assessed and improved using several tools. The reads of Avicennia marina was analyzed using miRanda to identify expressed miRNA (already annotated in miRBase) and novel miRNAs. Differential expression analysis was performed using DeepSeq Tool. Differentially expressed microRNAs under abiotic stress conditions were analyzed further.