CLSM reconstruction of bacteria in embryo tissue of deep-sea sponge H. (S.) methanophila, EUB probe

Catalyzed reporter deposition (CARD)-FISH with horseradish peroxidase-labeled probes (Biomers, Ulm, Germany), EUB338 (Amann et al.,1990) was performed on 8 μm sections of sponge tissue. Photomicrographs were acquired with confocal laser-scanning microscope (LSM 780, Carl Zeiss, Germany). Three channels were used - cy3, EUB probe, DAPI and 488 (autofluoresence). 3D representation of image stacks was reconstructed with Fiji 3D project option (interpolated)..