DATA SET FOR QUANTIFICATION OF TOTAL PHENOLIC COMPOUNDS, FLAVONOIDS, ANTIOXIDANT ACTIVITY AND IN VITRO ENZYMATIC INHIBITION ANDIROBA (CARAPA GUIANENSIS AUBL) INDUSTRIAL WASTE

This dataset aims to evaluate the antioxidant potential and the digestive enzyme inhibitory capacity of the ethanolic extract from Andiroba’s industrial residue. The ethanolic extract was obtained using a Soxhlet apparatus, and the concentrations of total phenols and flavonoids were determined. Antioxidant potential was evaluated using the DPPH and FRAP methods, while enzymatic activity was performed using the α-amylase and lipase enzymes. Additionally, a hemolytic assay was conducted to evaluate cytotoxicity. Data were expressed as mean ± standard deviation. The Student’s t-test was used for analysis of the results for total phenol/flavonoid content, antioxidant activity, and enzymatic activity. All assays were performed in triplicate. IC50 values were determined by non-linear regression with 95% confidence intervals, and p<0.05. GraphPad Prism version 6.0 was used for statistical analysis. The total phenol content was less than 1.7 µg GAE/mg and total flavonoids were 3.16 ± 2.67 µg QE/mg, close to the limit of quantification (8.37 µg/mL), indicating a low presence of these compounds in the sample. Regarding the extract's antioxidant potential (DPPH), it was observed that at a concentration of 10mg/mL, the sample demonstrated 41.24 ± 3.96% inhibition with an IC50 of 15.12 ± 3.01 µg/mL, indicating moderate antioxidant activity. In the FRAP method, the residue was able to inhibit 221.07 ± 5.82 µM of ferrous sulfate per mg of extract (µM ESF/mg), indicating that AIR has antioxidant potential. Furthermore, the Andiroba industrial residue demonstrated 65.38% inhibition of α-amylase enzyme activity at a concentration of 25 mg/mL, with an IC50 of 17.52 ± 4.57 mg/mL, and regarding lipase enzymatic activity, the inhibition was 53.17% at the same concentration mentioned above, with an IC50 of 19.68 ± 0.16 mg/mL