Table 1 in Colossoma macropomum (Characiformes: Serrasalmidae) adapted to new climate regime: differential gene expression from farmed tambaqui juveniles raised in subtropical and tropical regions

Table 1: Details of target genes (hif-1α, hsp70, ras, mstn, acly, per-1, cry-1, ube3a and ogt) and reference genes (β- tubulin and β- actin) primers.
GeneLength (bp)R 2Efficiency (%)Primers sequence (5ʹ-3ʹ) forward/reversetubulin -F200.99109.5GACGTGGTGCCCAAAGATGTtubulin -R18TGGATGGTGCGCTTGGTβ- actin -F210.99100.5GCTGTTTTCCCCTCCATTGTTβ- actin -R19TCCCATGCCAACCATCACThif-1α -F200.99105.2CTTCTGAGCTCTGATGAGGChif-1α -R20GAAAGCACCATCAGGAAGCChsp-70 -F200.99100.9GCAAGGAGAACAAGATCACChsp-70 -R19CACTCCGTTGCACTTGTCCmstn -F200.98100.5AATCCAAGCGAGGGAAAAGCmstn -R22CCTCCATCACCTGAAAGGTCTTras -F200.9799.31CCAGTACATGAGGACAGGAGras -R20CAAGCACCATTGGCACATCGacly -F190.99100.7ATCATCTCCCGCACTACAGacly -R19TACCTCCAATCTCTCCCAGube3a -F210.98103.3GCCATAAGCAAGCAGCACAACube3a -R19CCAGTCAGTCCGCACATCGper-1 -F200.98104.1TGTTGAAGTTTGTGCCCCAGper-1 -R18CAGTCCAGATGCTCCTCCcry-1 -F190.99103.6GTCCAACAGCCCTCAAACTcry-1 -R18TACGCCAAGCACTCCAGAogt -F190.99104.1CCTCCCTTTGCTGTGTTCCogt -R20TGTCTGCTTTCCGCTTTCGC