MeCP2 and H3K27me3 binding in Mecp2-knockout and MECP2-transgenic hippocampus. MeCP2 and H3K27me3 binding in Mecp2-knockout and MECP2-transgenic hippocampus

MeCP2 globally regulates gene expression in the brain. One example is Growth-differentiation factor 11 (Gdf11), which is down-regulated upon loss of MeCP2 and up-regulated upon gain of MeCP2. To assess if MeCP2 binds near the Gdf11 locus we performed CUT&RUN for MeCP2 in Mecp2-knockout or MECP2-transgenic hippocampus. To assess if a repressive histone modification (H3K27me3) was concordantly changed we profiled H3k27me3 in these same matched tissues. Overall design: CUT&RUN for MeCP2, H3K27me3, and Rabbit IgG from the nuclei of Mecp2-knockout, MECP2-transgenic, and associated wild-type littermates