Stress-regulated miR-708-5p affects recognition memory and manic-like behavior through Neuronatin downregulation.

microRNAs are posttranscriptional regulators that bind to target mRNAs. We tested the regulatory effect of miR-708-5p, a miRNA associated with Bipolar Disorder, in the mouse hippocampus. To unveil molecular mechanisms downstream of miR-708-5p, we stereotactically injected a virus overexpressing miR-708-5p for target discovery. We injected a miR-708-5p overexpressing virus or a control virus in the dorsal and ventral hippocampus of male wild type C57BL/6JRj mice, 7/8 weeks old. Four weeks post-surgery, we dissected the hippocampi and extracted RNA.