human gut metagenome Metagenome

We recruited 60 subjects over a 6-month period from the University of California San Diego undergraduate campus. Within this group of subjects were 24 separate households consisting of 2 individuals. We also enrolled 12 separate control subjects not enrolled with a housemate. In each household, 1 individual received treatment with an antibiotic, either amoxicillin (n=12) or azithromycin (n=12), and the other individual received treatment with a placebo (vitamin C, n=24). Antibiotic/placebo were given once per day with half the participants receiving therapy for 3 days and the other half for 7 days. The additional 12 subjects who were not enrolled with a housemate and did not receive either antibiotic or placebo (e.g., non-household controls). Fecal samples were collected on day 0 (day prior to antibiotics), day 3 (on the third day of antibiotics), day 7, week 8, and at 6 months.