Antimicrobial activity of chicken cathelicidins against a broad range of bacterial strains using spot-test.

All strains were human isolates unless otherwise noted.*: a) vancomycin resistant, b) methicillin resistant, livestock associated ST398, c) CTX-M-1 ESBL positive from chicken, d) TEM-52 -ESBL positive from chicken, e) NDM-1 carbapenemase positive, f) KPC carbapenemase positive, g) cystic fibrosis patients. Bacteria were cultivated overnight at 37°C, a suspension of bacteria (5×105 CFU/ml) in distilled water was prepared and spread over Müller-Hinton agar plates. After the plates were dried, 20 µl of a CATH solution (32 µM) was spotted, plates were cultured at 37°C and examined for growth inhibition by measuring the diameter of the zones and noting the presence or absence of colonies within the spot area. The table represents single or duplicate screening experiments.