Minimal dataset to test multiplexed DNA imaging (Hi-M) software pipelines

This is a dataset of nuclei (DAPI), and 3 multiplexed DNA imaging cycles to test and validate processing software packages, such as pyHiM (https://github.com/marcnol/pyHiM). This dataset was acquired in a nc14 Drosophila embryo. File contents: scan_001_RT27_001_ROI_converted_decon_ch00.tif barcode 27, fiducial <br> scan_001_RT27_001_ROI_converted_decon_ch01.tif barcode 27<br> scan_001_RT29_001_ROI_converted_decon_ch00.tif barcode 29, fiducial <br> scan_001_RT29_001_ROI_converted_decon_ch01.tif barcode 29 <br> scan_001_RT37_001_ROI_converted_decon_ch00.tif barcode 37, fiducial <br> scan_001_RT37_001_ROI_converted_decon_ch01.tif barcode 37 <br> scan_006_DAPI_001_ROI_converted_decon_ch00.tif DAPI <br> scan_006_DAPI_001_ROI_converted_decon_ch01.tif DAPI, fiducial <br> scan_006_DAPI_001_ROI_converted_decon_ch02.tif RNA To test this dataset please refer to pyHiM documentation page.