Genome-wide profiles of gene expression levels in mouse nephron progenitor cells

During embryonic kidney development, nephron progenitor cells (NPCs) self-renew and differentiate into all cells in mature nephrons. The Wnt signaling components Wnt9b and ß-catenin are required for both NPC self-renewal and differentiation. A low level of Wnt/ß-catenin are associated with NPC self-renewal while a high level with differentiation. To investigate the transcriptional mechanisms behind Wnt/ß-catenin-driven regulation of NPCs states, we modeled NPC self-renewal and differentiation in vitro with NPEM (Brown et al., 2015) supplemented with low (1.25 µM) or high (5 µM) concentration of CHIR22091 (CHIR), a small molecule GSK3ß inhibitor that stabilizes ß-catenin. To investigate change of NPC chromatin landscape under influence of CHIR treatment, here we generated ATAC-Seq data from primary NPC, as well as NPC cultured in low and high CHIR concentration. Overall design: RNA-Seq was performed in primary NPCs, as well as NPCs cultured in low (1.25 µM) CHIR and high (5 µM) CHIR conditions.