Mean generation time in BHI with CSP, presence of genetic competence, com genes and bacteriocin distribution among clinical isolates.

Mean generation times (Td) are same as in Table 1 (CSP). An * indicates Td is not significantly different from Td in BHI without CSP. The † for strains Smu69 indicates Td in CSP was significantly better (P≤0.003) with CSP compared to without CSP. Absence of com genes was determined by whole genome sequence analysis and confirmed by PCR for Smu56 and Smu57. ComC from Smu69 has a truncated comC gene, which is missing the last 3 C-terminal amino acids. For competence Y = yes and N = no. Putative bacteriocins: nlmA/B = Smu.150/Smu.151, nlmD = Smu.423, cipB = Smu.1914, Smu.1906. Putative immunity proteins: Smu.1909, Smu.1913, cipI = Smu.925, Smu.152. (Smu.1906),a = there appears to be a recombination between Smu.1914 and Smu.1906 resulting in a hybrid protein that contains the signal sequence of Smu.1914 and the bacteriocin sequence from Smu.1906. (NlmD),b = mutated signal sequence,c = truncated by 12 aa,d = insertion of two nucleotides resulting in a frameshift at amino acid 50. (Smu.925/CipI),e = ORF starts downstream at 54 bps ATG or GTG site,f = a frame-shift mutation results in a premature stop codon leading to a truncation of 4 aa at C-terminus,g = truncated by 7 aa at C-terminus due to a frame-shift mutation.