Whole transcriptome RNAseq analysis of germ cells in Inhba and Inha fetal testis

We performed bulk RNA-Seq on male fetal germ cells isolated from E13.5 and E15.5 testes from activin A-deficient mice (Inhba KO), and from E13.5 testes from mice with greater activin A bioactivity (Inha KO). The aim of this experiment was to determine how chronic altered activin A bioactivity altered the germline transcriptome. Germ cells were isolated from embryonic E13.5 and E15.5 Inhba x Oct4-Gfp WT and KO testes, and E13.5 Inha x Oct4-Gfp WT and KO testes via FACS. Cells were collected from n=3-4 (Inhba) and n=2 (littermate pairs; Inha) animals/age/genotype.本研究对来自激活素A缺陷型小鼠（Inhba KO）E13.5及E15.5天睾丸，以及激活素A生物活性增强小鼠（Inha KO）E13.5天睾丸的雄性胎儿生殖细胞开展批量RNA测序（bulk RNA-Seq）。本实验的研究目的为明确慢性激活素A生物活性改变如何调控生殖细胞的转录组。研究人员通过荧光激活细胞分选术（FACS），分别从胚胎期E13.5、E15.5天的Inhba×Oct4-Gfp野生型（WT）与敲除型（KO）睾丸，以及E13.5天的Inha×Oct4-Gfp野生型与敲除型睾丸中分离得到生殖细胞。每个组别、年龄与基因型对应的样本分别采集自n=3-4只（Inhba组）及n=2只（Inha组，同窝配对）实验动物。