analysis of allelic imbalance in Drosophila melanogaster F1 hybrids

Asymmetric expression of two alleles, also known as allelic imbalance (AI), that is observed between alleles present in the same cell (i.e., exposed to the same /trans/-regulatory environment) provides direct evidence of /cis/-regulatory differences. Here, we quantified allelic expression in cDNA pools derived from interspecific F_1 /Drosophila/ hybrids and used these data to test for significant AI (i.e., differences in expression between the maternal and paternal alleles).