RNA sequencing analysis of rat TMJ condylar chondrocytes under hypoxia and Mt1 knockdown

This RNA sequencing project aimed to explore the transcriptional responses of rat TMJ condylar chondrocytes to oxygen variation and Mt1 regulation. Primary condylar chondrocytes from 3 weeks old rats were cultured under physiological low oxygen (5% oxygen) and hypoxic (less than 1% oxygen) conditions, with or without Mt1 knockdown. Total RNA from each group was extracted and subjected to high-throughput sequencing using the Illumina HiSeq 4000 platform. The dataset provides a comprehensive transcriptomic resource for understanding how Mt1 modulates chondrocyte adaptation and metabolic homeostasis under hypoxic stress.