Sturgeon chondroitin sulfates inhibit the proliferation of colorectal cancer cells by inducing cell cycle arrest and apoptosis

Cell proliferation assay and flow cytometric analysis were used to examine the effects of SCS treatment on viability and apoptosis of colon cancer cell HT-29 cells and normal colonic epithelial cell NCM460 cells. Transcriptomic and proteomic studies were used to identify the main targets of SCS. SCS showed little effect on the gene expression profile of NCM460 cells whereas188 genes and 10 proteins were differentially regulated in HT-29 cells after SCS treatment. Enrichment analysis of those genes / proteins showed that majority of them are involved in DNA replication, cell cycle progression, and apoptosis. Construction of RNA-seq in HT-29 and NCM460 cells treated with chondroitin sulfate(SCS)