HCAR1 nuclear transportation is critical for cell function

Membrane receptor nuclear translocation play some novel role in cancer pathology. In the current studies, we demonstrate HCAR1 distributes in cancer cell nucleus in Lung cancer; Lactate promote HCAR1 nuclear translocation. Proteomics analysis found there are a set of nuclear proteins binds with HCAR1; interaction of HCAR1 with SFPQ etc other proteins promote cell self-renewal and cell invasion in lung cancer. ChIP sequencing analysis discovered there are a set of genes were targeted by HCAR1. Lung cancer cell with/without lactate treatment were used to immunoprecipitate chromatin fragments with anti-HCAR1 antibodies. The pellet then were used for ChIPseq.