Study of helminth eggs (Ascaris suum) inactivation by anaerobic digestion and electrochemical treatment

The use of insufficiently treated wastewater or fecal sludge in agriculture raises concerns because of the pathogen content. Helminth eggs are one of the most crucial pathogens for ensuring public health and safety. Widely used disinfection treatment methods do not guarantee the complete inactivation of helminth eggs. The current study evaluated the effectiveness of anaerobic digestion and electrochemical process for helminth (Ascaris suum) egg inactivation. Lab-scale biochemical methane potential (BMP) assay was conducted by spiking A. suum eggs in a serum bottle. Total solid (TS), volatile solid (VS), pH, biogas production and its composition, and volatile fatty acids (VFA) were analyzed along with A. suum inactivation, every 3rd day for the initial 15 days and 5th day till 45 days. The results documented 98% inactivation of A. suum eggs (0.15 eggs/ml) in 35 days and remained at 0.14 eggs/ml till the 45th day. Correlation analysis revealed positive relation of non-viable eggs with pH ..., The manuscript contains comprehensive information on data collecting, and the README.txt file contains detailed descriptions of each data file, which are briefly outlined here:
1. METHODS
Anaerobic Digestion- BMP (Biochemical Methane Potential) Assay
1.1 Experimental setup
The BMP assay was performed in serum bottles for the co-digestion of faecal sludge and food waste. Each bottle was subjected to a loading rate of biomass of 1.5kg VS/m3. Macro and micronutrients, Na-bicarbonate, digested from an anaerobic pilot reactor was used as inoculum, and the final volume was raised to 100ml by adding distilled water. A. suum eggs were procured from Excelsior Sentinel, USA, a mixture of viable and non-viable eggs, and spiked into the bottles. The experiment was performed using two bottles in each set; one bottle from each set was taken out at each sampling point for analysis, and another bottle along with the remaining bottles from the set was used to take the gas reading every day. The setup w..., For a description of the uploaded data, files, and script, see README.txt.
In summary, there are total twelve data files. 
Files pH-PP and SM_CSV, Total Solids (TS) and Volatile Solids (VS)_PP and SM_CSV, Volatile Fatty Acids (VFA)_PP and SM_CSV, Biogas_PP and SM_CSV, Biogas composition (CH4, CO2 and H2S)_PP and SM_CSV, A. suum_PP and SM_CSV contains details of pH, TS VS, VFA, Biogas, Biogas composition, and Ascaris suum data respectively. File number seven [A.suum statistical data  file_PP and SM_CSV] contains data used for statistical significance and correlation analysis. File number eight [A. suum stat data output file_PP and SM] is output of the statistical significance and correlation data. File number nine and ten README.txt File_PP and SM and README.md contains information on methodology and experimental setup. File eleven and twelve Supporting figures_PP and SM and Supporting tables_PP and SM contain figures and tables respectively.Â