Sperm length and seminal fluid proteins promote male reproductive success in D. melanogaster

Spermatozoal morphology is highly variable both within and among species, often corresponding to variation in the shape of the female sperm storage organs in ways that can significantly impact fertilization success. In an effort to understand genetic mechanisms of sperm length variation, we compared gene expression patterns in the testes of Drosophila melanogaster males that produce either long or short sperm. We found that genes upregulated in long sperm testes are enriched for long noncoding RNAs (lncRNAs) and seminal fluid proteins (Sfps). Transferred in seminal fluid to the female during mating, Sfps are secreted by the male accessory glands and affect female remating rate, physiology, and behavior with concomitant advantages for male reproductive success. While sperm and Sfps are both critical for male reproductive success, they are largely considered to be functionally, genetically, and developmentally independent and despite being upregulated in long sperm testes, Sfps have no kn..., , # Sperm length and seminal fluid proteins promote male reproductive success in *D. melanogaster*

Dataset DOI: [10.5061/dryad.gb5mkkx2j](10.5061/dryad.gb5mkkx2j)

## Description of the data and file structure

# README for Datasets Associated with Galvin J, EL Larson, S Yedigarian, M Rahman, K Borziak, M DeNieu, and MK Manier

**Title:** Genetic coordination of sperm morphology and seminal fluid proteins promotes male reproductive success in *Drosophila melanogaster*

## Experiment 1: RNAseq of Testes Producing Long or Short Sperm

### Datasets:

* **Supp1_rpkm_all.csv**
* **Supp2_DEgene.summary.csv**
* **rpkm.sfps.csv**

### **Supp1\_rpkm\_all.csv**

| Column | Description |
| :--------------------------------------------- | :--------------------------------------------------------------- |
| ID | FBgn gene identifier ...,