Ecuadorian Dendrobatid frog alkaloid profiles

Shifts in microbiome community composition can have large effects on host health. It is therefore important to understand how perturbations, like those caused by the introduction of exogenous chemicals, modulate microbiome community composition. In poison frogs within the family Dendrobatidae, the skin microbiome is exposed to the alkaloids that the frogs sequester from their diet and use for defense. Given the demonstrated antimicrobial effects of these poison frog alkaloids, these compounds may be structuring the skin microbial community. To test this, we first characterized microbial communities from chemically defended and closely related non-defended frogs from Ecuador. Then we conducted a laboratory experiment to monitor the effect of the alkaloid decahydroquinoline (DHQ) on the microbiome of a single frog species. In both the field and lab experiments, we found that alkaloid-exposed microbiomes are more species rich and phylogenetically diverse, with an increase in rare taxa. To ..., , , # Ecuadorian Dendrobatid frog alkaloid profiles

[https://doi.org/10.5061/dryad.9ghx3ffqn](https://doi.org/10.5061/dryad.9ghx3ffqn)

Alkaloid GC-MS data from frog samples. GC-MS analysis was performed on a Shimadzu GCMS-QP2020 instrument with a Shimadzu 30m x 0.25 mmID SH-Rxi-5Sil MS column. The GC-MS data files were exported as CDF files and the Global Natural Products Social Network (GNPS) was used to perform the deconvolution and library searching against the AMDIS (NIST) database to identify all compounds ([https://gnps.ucsd.edu](https://gnps.ucsd.edu/)).

The Shimadzu LabSolutions GCMS software was used to generate the CDF files, but they can also be opened with publicly available software MZmine 3: [http://mzmine.github.io/](http://mzmine.github.io/) along with other similar open-source software.

## Sample Metadata

See file `Alkaloid_metadta.csv`

* **filename**: Name of GCMS file
* **ATTRIBUTE_species**: Frog species
* **ATTRIBUTE_population**: Frog geographic location
* **ATTR...