Conservation of transport proteins and their substrates across five Asgard proteomes.

The first 2 columns show the TC IDs (TCIDs) and protein accessions (Accessions) of the homologs in TCDB that were used to annotate the candidate transporters identified in the Asgard proteomes. The third and fourth columns describe the substrates transported by the TC protein based on the ChEBI ontology [170] accessions for chemical entity (ChEBI_chemical_entity) and biological role (ChEBI_role), respectively. The fifth column (TC_hit_tms_number) shows the number of TMSs inferred by HMMTOP [46] in the TC protein. Columns 6–10 show the presence (+) or absence (-) of a given TC system in the proteomes of Odin, Thor, Heimdall, Loki and MK-D1. Columns 11–16, 17–22, 23–28, 29–34 and 35–40 contain information describing the alignments between TC proteins (i.e., the hits) and the top matches from Asgard candidate transporters (i.e., the queries) in Odin, Thor, Heimdall, Loki and MK-D1, respectively. These 6-column blocks show for each proteome the accession of the corresponding Asgard protein (query), the number of HMMTOP-predicted TMSs (q_tms) in the Asgard protein, the Smith-Waterman E-value (evalue) of the alignment as implemented in SSEARCH [171], the percent identity (pident), and the alignment coverages for both the Asgard (qcov) and TC proteins (scov). (XLSX)