Astrocytes proliferation and counting living cells.
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Astrocytes were isolated and seeded at 7x104 cells/35 mm dish during 5 days. At this time, cells were incubated without Rn (control, C) or with Rn (10–7, 10–6, 10–5 M) for 24 h. Trypan blue exclusion was used to count the living cells and monitor cell proliferation. Data are mean ± SD of four independent experiments (four different rats).
创建时间:
2016-09-29



