Melting point evaluation of TINA modified oligonucleotides.

Change in Tm and ΔTm of Watson-Crick based antiparallel duplexes stabilized by para-TINA (X) and/or ortho-TINA (Y) molecules. Tm was determined using 0.5 µM of each strand in 50 mM phosphate buffer, pH 7.0, with 100 mM NaCl and 0.1 mM EDTA. Tm was defined as the peak of the first derivate using both annealing and dissociation curves. Base mismatches are underlined and marked in bold black. *Mismatch adjacent to TINA.