Methylation data from nine tissues from GTEx samples profiled with Infinium HumanMethylationEPIC BeadChip

Genome wide DNA methylation profiling of tissue samples from GTEx donors. The Infinium HumanMethylationEPIC BeadChip was used to obtain DNA methylation profiles across approximately 850,000 CpGs sites from 1000 tissue samples. Epigenome-wide DNAm was derived from 1,000 tissue samples from 9 unique tissue types - breast mammary tissue, colon transverse, kidney cortex, lung, muscle skeletal, ovary, prostate, testis and whole blood - obtained from 424 GTEx subjects, mostly (~83%) from European ancestry given available genotype data and the majority self-identified as white. DNA samples were extracted from GTEx tissue samples using Qiagen Gentra Puregene method at GTEx Laboratory Data, Analysis and Coordinating Center (LDACC), and sent to the Institute for Population and Precision Health Laboratory on 96-well plates. Tissue types were not batched by plate. Bisulfite conversion was applied to 500 ng of DNA using EZ-96 DNA methylation kit (Zymo Research, Irvine, CA, USA). All samples were then prepared and analyzed in accordance with the manufacturer guidelines and protocol for the Infinium MethylationEPIC array (Illumina, San Diego, CA, USA); which was utilized to measure DNAm levels for 866,895 (867K) genomic locations, encompassing primarily CpG dinucleotides but also non-CpG sites. >>>Submitter states: the IDAT files are currently available at AnVIL/dbGaP (phs000424.v9). By GTEx consortium policy, IDAT files cannot be made publicly available as they could be used to re-identify GTEx samples <<<