Nkx2.7 is a Conserved Regulator of Craniofacial Development

Pharyngeal arch cells are comprised of endoderm, mesoderm, and neural crest layers. We used single cell RNA sequencing (scRNA-seq) to analyze the genetic differences in pharyngeal arch cell types between wild-type and nkx2.7-/- samples. Pharyngeal arch cells were obtained through manual dissection of wild-type and nkx2.7-/- embryos at 26 hpf. All embryos were positive for TgBAC(tcf21:mcherry-NTR), which was used as a visual guide for dissection. Cells were FACS sorted to eliminate dead cells from sequencing. 2 wild-type embryos and 2 nkx2.7-/- samples were pooled for submission.