Nuclear/mitochondria genome exchange and one-step isogenesis through non-canonical mitosis

We sought to create a set of strains that each contain a nuclear genome entirely derived from the male parent, and a mitochondrial genome entirely derived from the maternal parent. We chose to utilize a wild strain (PX174) that has diverged sufficiently from the wildtype lab reference strain (N2) to allow a definitive assessment of chromosome-wide derivation throughout the genome.