VenomSeq on 9 purified teretoxin peptides

# DESCRIPTION OF CONTENTS <br> This directory contains one CSV file for each of 13 expression signatures (9 experimental samples and 3 controls) used to evaluate the VenomSeq technology platform against isolated, purified venom components. <br> <br> # OTHER EXPERIMENTAL INFORMATION <br> The human cell line used for perturbational differential expression analysis is IMR-32. <br> <br> # OTHER INCLUDED FILES <br> Original raw data is provided in the data/ subdirectory. <br> Also included is an R-Markdown file used to perform the differential expression analysis. <br> <br> # EXPLANATION OF DIFFERENTIAL EXPRESSION SIGNATURES <br> An empty CSV file indicates that no genes were significantly differentially expressed on exposure to the peptide in question. <br> Two positive controls are included: <br> - Cy3crotamine.csv; Fluorescent version of crotamine, a major peptide from the venom of Crotalus durissus terrificus known to inhibit the growth of several cancer cell types via the inhibition of voltage-gated potassium ion channels such as Kv1.3. Since crotamine is known to have strong effects on human neuronal cell lines (such as IMR-32), we expected to see a robust differential expression signature in the form of multiple up- and down-regulated genes. This is confirmed by Crotamine yielding the largest expression signature. <br> - Cimperialis.csv; Conus imperialis - the magician cone snail - is a venomous marine snail with a complex venom consisting of several hundred distinct components. Crude C. imperialis venom was used in the inital run of VenomSeq on venoms from 25 diverse species. The resulting expression signature in this VenomSeq run is consistent with the signature produced in the original run. <br> One negative control is included: <br> - GsMTx4.csv; GsMTx-4 is a neurotoxic venom component from Grammostola spatulata, which reduces sensation by inhibiting mechanosensitive channels such as PIEZO1 and TRPC6. This is a negative control, because these inhibitory effects are not expected to result in significant changes to gene expression. This is confirmed by the signature being empty. <br> <br> Expression signatures for 9 teretoxins selected from an in-house terebrid venom component library are included: <br> - Tba1.1 - Tv1 - Tan9.10 - Tfu6.8 - Ts14.1 - Mki8.7 - Tan22.12 - Tsu1.1 - ArgT5 <br> Mki8.7 is the teretoxin yielding the most robust differential expression signature, with 13 down-regulated and 12 up-regulated genes. <br> 5 teretoxins yielded empty expression signatures. <br>