Identification of microbiota of Drosophila populations evolved on poor diet compred to control populations

Six outbred Drosophila melanogaster populations (Selected) have been maintained on a poor diet for >170 generations, anotoher six populations (Control) have been maintained in parallel on standard diet. We used 16S rRNA gene sequencing (regions V1-V2) to compare the composition of their microbiota, both in their "conventional" state (as they are maintained on the respective diets in the experimental evolution) and under experimental inoculation with a standard mixed feces inoculum. The detailed methods and results are reported in: Erkosar, B., S. Kolly, J. R. van der Meer, and T. J. Kawecki. Adaptation to chronic nutritional stress leads to reduced dependence on microbiota in Drosophila melanogaster, mBio 8:e01496-17.