Method of identifying a mutant plant
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
In traditional plant breeding approaches， chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant， i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities， the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention， however， demonstrates how a novel， alternative use of digital polymerase chain reaction (dPCR)， preferably droplet dPCR (ddPCR)， is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms， digital PCR-based systems and a set-up to propagate and analyze identified， mutated organisms.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
