Glucose metabolism drives epigenetic landscape transitions that dictate muscle stem cell function

We report the application of chromatin immunoprecpitation (ChIP) and assay for transposase accessible chromatin (ATAC) followed by sequencing to assay chromatin response to metabolic perturbation. Primary myoblasts were treated with dichloroacetate (DCA) for 24 hours, which increases the contribution of glucose to histone acetylation, as well as overall acetylation. ChIP and ATAC-seq was performed to establish the genomic specificity of this response, as well as the resulting effect on chromatin accesibility.