Identification of Drosophila TDF/Apt regulated genes in embryos. Drosophila melanogaster

Drosophila tdf, another name apontic (apt), encodes a bZIP transcription factor that is required for the development of trachea, heart, head and neural system. However, little is known about the target of TDF/Apt. To identify theTDF/ Apt regulated genes, we compared expression profiles of mRNA purified from wild-type and tdf mutant embryos. Summary file is emtdf-yw.CHP. Overall design: yw and tdf mutant embryos were selected at later stage of development for RNA extraction and hybridization on Affymetrix microarrays. We identified tdf mutant embryos using CyOact-GFP balancer chromosome.