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Single cell transcriptome analysis of human oocyte aging

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE155179
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To investigate the effects of women age on oocyte developmental competency, we used single-cell RNA sequencing to detect global gene transcriptome and identify key genes affected by advanced age in human mature oocytes. We isolated mRNA from MII oocytes obtained from ICSI patients (six oocytes from younger (<30 years) and six oocytes from older (≥40 years) patients for scRNA-seq.
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2021-05-27
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