Data from: Dietary regulation of the gut microbiota engineered by a minimal defined bacterial consortium

We have recently reported that Altered Schaedler Flora (ASF) can be used
to durably engineer the gut microbiota to reduce ammonia production as an
effective modality to reduce morbidity and mortality in the setting of
liver injury. Here we investigated the effects of a low protein diet on
ASF colonization and its ability to engineer the microbiota. Initially,
ASF inoculation was similar between mice fed a normal protein diet or low
protein diet, but the outgrowth of gut microbiota differed over the
ensuing month. Notable was the inability of the dominant Parabacteroides
ASF taxon to exclude other taxa belonging to the Bacteroidetes phylum in
the setting of a low protein diet. Instead, a poorly classified yet highly
represented Bacteroidetes family, S24-7, returned within 4 weeks of
inoculation in mice fed a low protein diet, demonstrating a reduction in
ASF resilience in response to dietary stress. Nevertheless, fecal ammonia
levels remained significantly lower than those observed in mice on the
same low protein diet that received a transplant of normal feces. No
deleterious effects were observed in host physiology due to ASF
inoculation into mice on a low protein diet. In total, these results
demonstrate that low protein diet can have a pronounced effect on
engineering the gut microbiota but modulation of ammonia is preserved.