Potential modulation of Puf3 chromatin association mediated by Rpb4

Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, a crosstalk where mRNA decay machinery impacts transcription and transcription machinery influences mRNA stability. Rpb4, and likely the dimer Rpb4/7 seem to be the central components of the RNA pol II governing these processes. Here we investigate the effect of ∆rrp4 on the potential chromatin association of the RNA binding protein Puf3 to chromatin in S. cerevisiae. ChIP-seq was performed for 8 samples: 3 biological replicates for PUF3-TAP WT and PUF3-TAP Δrpb4 strains, and 2 biological replicates for the WT strain. For each sample, input was also sequenced.