Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity

We identify clearly distinct transcriptomic signatures between clear cells from the caput, corpus and cauda regions, which might reflect a more accurate functional characterization of a given specific cell type. Overall design: Clear cell gene-expression profile based on their location in the epididymis using RNA-sequencing. EGFP+ clear cells were isolated by double cell sorting from the caput, corpus and cauda epididymal regions of B1 V ATPase - EGFP transgenic mice that specifically express EGFP in clear cells.