Supporting data for "The role of ACSS2 in early transcriptional regulation during reversible induction"

This dataset contains raw and processed data for ChIP-seq, chrRNA-seq, IFA, qPCR, Western blot, and other analysis used in the thesis entitled "The role of ACSS2 in early transcriptional  regulation during reversible induction".

Histone acetylation is an active histone mark for active enhancers or promoters, promoting gene expression. The process is catalysed by lysine acetyltransferases, which are able to bind to specific DNA sequences via transcription factors, and transfer the acetyl-group from acetyl-CoA to lysine residues of histone tails. Recently, there is evidence suggesting that acetyl-CoA providing enzymes may have specific chromatin-binding ability to regulate gene expression as well. 

In this project, we will dissect the role of acyl-CoA synthetase short-chain family member 2 (ACSS2) in transcriptional reprogramming of reversible inductions, with glucocorticoid signaling as an example using high-throughput technologies. The specific chromatin-binding patterns and transcription modulating effect of ACSS2 will first be investigated by ChIP-seq and chrRNA-seq techniques respectively. The interplay and recruitment dynamics between ACSS2, other histone modifying enzymes and transcription factors will be compared to understand the cascade of transcription activation. In summary, this thesis comprehensively evaluates the temporospatial changes and functional impacts of ACSS2 in early glucocorticoid receptor signalling..

本数据集囊括了 ChIP-seq、chrRNA-seq、免疫荧光分析（IFA）、定量PCR（qPCR）、蛋白质印迹（Western blot）等多种分析所使用的原始及处理后的数据。这些分析手段用于探究题为《ACSS2 在可逆诱导早期转录调控中的作用》的论文。组蛋白乙酰化作为一种活跃的组蛋白标记，对于增强子或启动子的激活具有促进作用，进而推动基因表达。此过程由赖氨酸乙酰转移酶催化，这些酶能够通过转录因子与特定的 DNA 序列结合，并将乙酰基从乙酰辅酶 A 转移至组蛋白尾部的赖氨酸残基。近期的研究表明，提供乙酰辅酶 A 的酶可能具有特定的染色质结合能力，以调节基因表达。在本项目中，我们将利用高通量技术，以糖皮质激素信号传导为例，深入剖析酰基辅酶 A 合成酶短链家族成员 2（ACSS2）在可逆诱导转录重编程中的作用。我们将通过 ChIP-seq 和 chrRNA-seq 技术分别研究 ACSS2 的特异性染色质结合模式和转录调节效应。比较 ACSS2 与其他组蛋白修饰酶以及转录因子之间的相互作用和招募动力学，以理解转录激活的级联反应。总之，本论文全面评估了 ACSS2 在早期糖皮质激素受体信号传导中的时空变化及其功能影响。