Spontaneous or DSB-stimulated interchromosomal recombination and plating efficiencies in wild type and homozygous mutant diploid strains.

aAll diploid strains were homozygous for MATa::LEU2 such that no cutting by HO endonuclease occurred at the MAT locus, except for T0 strains where HO endonuclease is not expressed.
bFrequencies of ectopic gene conversion (EGC) in diploid cells between sam1-ΔBglII at the SAM1 locus on one copy of chromosome XII and sam1-ΔSalI at the HIS3 locus on one copy of chromosome XV were determined following a HO endonuclease-mediated break in sam1-ΔBglII as described in the Materials and Methods. Median frequencies were determined from a minimum of 10 independent cultures for each strain. Fold differences from the median frequency observed with wild type strains are indicated in brackets with fold increases preceded by a “plus” and fold decreases preceded by a “minus“. The 95% confidence intervals are indicated in parentheses. Frequencies in wild type and rad52Δ/rad52Δ homozygotes described previously [49].
cRates of translocation formation in diploid cells by spontaneous HR (T0) between a 300 bp segment of the HIS3 coding sequence shared by the his3-Δ5′ substrate at the LEU2 locus on one copy of chromosome V and a his3-Δ3′ substrate at the HIS3 locus on one copy of chromosome XV were determined using the method of the median from a minimum of 10 independent trials as described in the Materials and Methods. Fold differences from wild type and 95% confidence intervals are displayed as described above. ND  =  not determined. These results were published previously [27].
dFrequencies of translocation formation in diploid cells by HR between a 300 bp segment of the HIS3 coding sequence shared by the his3-Δ5′ substrate at the LEU2 locus on one copy of chromosome III and a his3-Δ3′ substrate at the HIS3 locus on one copy of chromosome XV were determined following a HO endonuclease-mediated break adjacent to the his3-Δ5′ substrate (T1) as described in the Materials and Methods. Median frequencies, fold differences from wild type and 95% confidence intervals are displayed as described above.
eFrequencies of translocation formation in diploid cells by HR between his3-Δ5′ and his3-Δ3′ following HO endonuclease-mediated breaks adjacent to both substrates (T2) were determined as described in the Materials and Methods. Median frequencies, fold differences from wild type and 95% confidence intervals are displayed as described above.
fPlating efficiencies (PE) “Pre” and “Post” HO endonuclease cutting at his3-Δ5′ and his3-Δ3′ were determined as described in the Materials and Methods, and previously. Median frequencies, fold differences from wild type and 95% confidence intervals are displayed as described above.