Crystal structure of EndoSz mutant D234M, in space group P21, in complex with oligosaccharide G2S1

Crystal structure of EndoSz mutant D234M, in space group P21, in complex with oligosaccharide G2S1 Descriptor: CALCIUM ION, Glycoside hydrolase, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Authors: Guan, H.H, Lin, C.C, Hsieh, Y.C, Chen, C.J. Deposit date: 2023-08-24 Release date: 2024-07-03 Last modified: 2024-07-10 Method: X-RAY DIFFRACTION (3.1 Å) Cite: Structure-Based High-Efficiency Homogeneous Antibody Platform by Endoglycosidase Sz Provides Insights into Its Transglycosylation Mechanism. Jacs Au, 4, 2024空间群为P2₁的EndoSz突变体D234M与寡糖G2S1结合的复合物晶体结构。配体描述：钙离子（CALCIUM ION）、糖苷水解酶（Glycoside hydrolase），以及目标寡糖序列：N-乙酰-α-神经氨酸（N-acetyl-alpha-neuraminic acid）-(2→6)-β-D-半乳糖吡喃糖（beta-D-galactopyranose）-(1→4)-2-乙酰氨基-2-脱氧-β-D-葡萄糖吡喃糖（2-acetamido-2-deoxy-beta-D-glucopyranose）-(1→2)-α-D-甘露吡喃糖（alpha-D-mannopyranose）-(1→3)-[β-D-半乳糖吡喃糖（beta-D-galactopyranose）-(1→4)-2-乙酰氨基-2-脱氧-β-D-葡萄糖吡喃糖（2-acetamido-2-deoxy-beta-D-glucopyranose）-(1→2)-α-D-甘露吡喃糖（alpha-D-mannopyranose）-(1→6)]β-D-甘露吡喃糖（beta-D-mannopyranose）-(1→4)-2-乙酰氨基-2-脱氧-β-D-葡萄糖吡喃糖（2-acetamido-2-deoxy-beta-D-glucopyranose）。作者：Guan, H.H、Lin, C.C、Hsieh, Y.C、Chen, C.J. 提交日期：2023年8月24日，发布日期：2024年7月3日，最后修改日期：2024年7月10日。解析方法：X射线衍射（X-RAY DIFFRACTION），分辨率3.1 Å。引用文献：《Structure-Based High-Efficiency Homogeneous Antibody Platform by Endoglycosidase Sz Provides Insights into Its Transglycosylation Mechanism》，发表于Jacs Au，第4卷，2024年。