Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast. Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

For the first time in any system, we generated experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from un-arrested, growing, and synchronously dividing yeast cells. Overall design: We used centrifugal elutriation to obtain our synchronous cell cultures. From ~100 different elutriated cultures, we generated a cell size-series, spanning a range from 40 to 75 fL, sampled approximately every 5 fL intervals, in three biological replicates in each case. The same 24 distinct pools were aliquoted as needed to generate the input samples for measurements of RNA (with RNAseq), proteins (with LC MS/MS), and metabolites (GC-TOF MS for primary metabolites; HILIC-QTOF MS/MS for biogenic amines; and CSH-QTOF MS/MS for lipids). Here, we deposit the RNA datasets.