Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.

FishPeakAreaPeak area calculated by Genemapper from ABI sequencer chromatograms for European minnow samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.FishPeakHeightPeak height in Relative Fluorescent Units (RFU) calculated by Genemapper from ABI sequencer chromatograms for European minnow samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.RicePeakAreaPeak area calculated by Genemapper from ABI sequencer chromatograms for rice samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.RicePeakHeightPeak height in Relative Fluorescent Units (RFU) calculated by Genemapper from ABI sequencer chromatograms for rice samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.