Cohesin occupancy and composition at enhancers and promoters are linked to DNA replication origin proximity in Drosophila. Cohesin occupancy and composition at enhancers and promoters are linked to DNA replication origin proximity in Drosophila

Cohesin subunits, the Nipped-B cohesin loader, the MED1 and MED30 subunits of the Mediator complex, and the Fs(1)h BET domain protein were mapped by ChIP-seq in ML-DmBG3-c2 cells and their effects on each other's association with promoters, enhancers, Polycomb Response Elements, and DNA replication origins were measured. DNA replication in early S phase was measured by ChIP-seq and correlated with functional element occupancy by cohesin. Overall design: Occupancy of functional features by different cohesin subunits and Nipped-B were compared, the effects on cohesin subunit and Nipped-B occupancy when different cohesin subunits and MED30 were depleted by RNAi relative to mock-treated controls were measured. The effects on cohesin subunit and Nipped-B occupancy when Fs(1)h chromosome binding was inhibited by the JQ1 inhibitor were measured relative to mock-treated controls.