RNA sequencing of adult cardiomyocyte cyclin C deletion

The goal of this study was to identify differentially expressed genes in hearts with cardiomyoycte deletion of cyclin c compared to cyclin c floxed controls. Overall design: RNA from three, six week old male hearts from cyclin C fl/fl and cyclin C fl/fl; aMHC cre was isolated. The University of Iowa Institute of Human Genetics, Genomics Division, generated polyA-enriched stranded RNA libraries followed by RNAseq using the Illumina HiSeq platform. Raw sequence reads were analyzed with BaseSpace (www.illumina.com) by aligning reads to the Mus musculus mm10 genome using the TopHat Alignment app. Transcripts were assembled and significant differentially expressed genes were determined with the Cufflinks Assembly and DE app using a false discovery rate <0.05.