Analysis of exosomal miRNAs in MCF7 cells regulated by LSD1. Analysis of exosomal miRNAs in MCF7 cells regulated by LSD1

Lysine specific demethylase 1 (LSD1) is an important histone demethylase that mediates metastasis in luminal breast cancer. Exosomes play a major role in cell-to-cell communication. Thus, the characterization of miRNAs of exosome in MCF7 cells regulated by LSD1 is imperative in clarifying intercellular signaling. Overall design: miRNA-seq in exosomes isolated from scrambled control siRNAs transfected MCF7 cells (NC), LSD1-specific siRNAs transfected MCF7 (KD), MCF7 cells transfected with LSD1-specific siRNAs and LSD1 rescue plasmid (RE). Each cell type have 3 biological replicates.