Comparison analysis of transcriptome profiles between tumor cells before or after immune editing

To gain insight into the differential expressed genes in immune edited tumor cell lines, we performed genome-wide RNA-sequencing analysis (RNA-seq) in parental P0 (CaSki-P0) and immune edited P3 (CaSki-P3) cells. We established a highly immune-resistant cervical cancer cell line, CaSki P3 (termed P3), which was generated from its immune susceptible parental cell line CaSki P0 (termed P0) through three rounds of in vitro selection by mixing P0 cells pulsed with the MART-1 peptide together with MART-1-specific CTLs.