A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome. Homo sapiens

Transcriptome of HEK and B cells were analyzed by microarray and RNA-Seq parallely. Both platforms were then compared in terms of sensitivity. To assess whether values were a reliable indicator of gene activity, we correlated these values with hypophosphorylated RNA polymerase II (PolIIa) occupancy, used as a landmark of transcription initiation. For HEK, we identified PolIIa islands by chromatin immunoprecipitation and sequencing (ChIP-Seq). Overall design: For HEK and B cell, samples were hybridized as biological and technical duplicates on Illumina HumanRef8 V2.0 BeadChips. PolIIa Chip-Seq performed on HEK.