Gene expression data from Tyr::CreErT2::BrafV600E Pten F/+ mouse tumors according to defloxing status of Brn2 [cell lines]

To investigate the mechanism underlying the effect of Brn2-loss we extracted mRNA from Braf-Pten-Brn2-wt, Braf-Pten-Brn2-het, and Braf-Pten-Brn2-hom mouse melanomas and performed microarray-based transcriptome analysis. We studied the effect of Brn2 loss on in vivo melanomagenesis by generating the mouse cell lines (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2+/+) = (Braf-Pten-Brn2-wt), (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/+) = (Braf-Pten-Brn2-het) and (Tyr::CreERt2/°; BrafV600E/+; PtenF/+; Brn2F/F) = (Braf-Pten-Brn2-hom) by introducing the floxed Brn2 locus into the genome by appropriate crossings (Figure 2 S2A) {Jaegle, 2003 #1672}. Cre-mediated defloxing of Braf, Pten, and Brn2 loci was induced by topical application of tamoxifen during the first three days after birth (Figure 2 S2B). All mice were monitored for the number of tumors/mice, and the growth rate of the first tumor. Cell lines were established from these tumors