Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
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Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,...
学界普遍认为，用于测序的聚合酶链式反应（Polymerase Chain Reaction，PCR）扩增应采用混合平行反应体系，以降低由jackpot效应（jackpot effects）与嵌合体形成（chimera formation）所引发的扩增偏差。然而，……
