Yagi et al. Figure data

The files contain the data underlying the figures in: Moeri Yagi, Simon Miller, Yoshiko Nagai, Shinsuke Inuki, Ayato Sato, and Tsuyoshi Hirota, A methylbenzimidazole derivative regulates mammalian circadian rhythms by targeting Cryptochrome proteins. <em>F1000Research</em> Figure 1B.csv (Luminescence rhythms of <em>Bmal1-dLuc</em> and <em>Per2-dLuc</em> U2OS cells in the presence of various concentrations of TH401 (<em>n</em> = 2)) Figure 1C.csv (Changes in period (<em>n</em> = 6)) Figure 1D.csv (Changes in luminescence intensity (<em>n</em> = 6)) Figure 1E.csv (Changes in cellular ATP levels after treatment with various concentrations of TH401 (<em>n</em> = 4)) Figure 2A.csv (Inhibitory effect of Longdaysin and TH401 on CKIδ activity <em>in vitro</em> (<em>n</em> = 1)) Figure 2B.csv (Changes in the half-lives of CRY-luciferase fusion proteins (CRY1-LUC and CRY2-LUC) relative to LUC in the presence of various concentrations of TH401 (<em>n</em> = 2)) Figure 2C.csv (Changes in <em>Per2::Luc</em> knock-in reporter activity in wild type, <em>Cry1/Cry2</em> double knockout, <em>Cry1</em> knockout, and <em>Cry2</em> knockout fibroblasts (<em>n</em> = 4–8)) Figure 2D.csv (Changes in the cellular circadian period of <em>Bmal1-Eluc</em> reporter rhythms in <em>Cry1/Cry2</em> double knockout fibroblasts rescued with CRY1 and CRY2 (<em>n</em> = 3–6)) Figure 2E.csv (Changes in the protection of CRY1 and CRY2 proteins from thermal denaturation in HEK293T cells (<em>n</em> = 4)) Figure 2F.csv (Changes in denaturing temperatures of recombinant CRY1(PHR) and CRY2(PHR) <em>in vitro</em> (<em>n</em> = 2)) Figure 4.csv (Changes in the circadian period in primary screening (tested at 7 μM; <em>n</em> = 1) and a secondary assay (tested at 24 or 8 μM; <em>n</em> = 3)) <br>