Proprioceptive limit detectors mediate sensorimotor control of the Drosophila leg

The datasets in this repository were used to reveal that hair plate proprioceptors on the legs of fruit flies are limit detectors that reflexively transition the direction of leg movement. We first collected a calcium imaging dataset to characterize the joint angle encoding properties of one hair plate on the fly coxa, referred to as CxHP8. We then used an electron microscopy dataset of a female ventral nerve cord (FANC) to predict the role of CxHP8 neurons in leg motor control based on their direct and indirect connectivity with motor neurons. We validated the predictions derived from the connectome by collecting and analyzing datasets where we optogenetically activated CxHP8 neurons in standing flies and silenced CxHP8 neurons in behaving flies. Additionally, we collected datasets in which flies walked on an actuated treadmill and found that chronic silencing of CxHP8 neurons lead to impaired walking kinematics and resting posture. Having supported the sensorimotor control prediction ..., Calcium imaging to characterize joint angle encoding properties
To determine the coxa joint angles in which CxHP8 neurons were active, we expressed GCaMP7f and tdTomato in them and slowly moved the platform that the front legs rested on using a 3-axis micromanipulator. We used a 2-photon microscopy setup to image the calcium activity of CxHP8 axons in the neuropil associated with the front left leg. tdTomato was used for motion correction and normalization. In addition to characterizing the joint angles encoded by CxHP8 neurons, we also determined when they are active during locomotion, grooming, and other behaviors. Analyses were done in the Jupyter notebooks, “calcium_imaging_analysis.ipynb” and “analyze_calcium_signals_during_behavior.ipynb”, found at this GitHub repository release: https://github.com/Prattbuw/Hair_Plate_Paper/releases/tag/v1.0.0. The relevant dataset is “R48A07_R20C06_GCaMP7f_tdTomato.parquet”.
Hair plate reflex circuit connectivity
Hair plate connectivity with moto..., # Proprioceptive limit detectors mediate sensorimotor control of the Drosophila leg

Dataset DOI: [10.5061/dryad.fxpnvx153](10.5061/dryad.fxpnvx153)

## Data Description and File Structure

### Opening Parquet Files

Parquet files were read using the Python Pandas library. Specifically, the data within parquet files were accessed using the following function: pd.read_parquet(\"file path\" + \"filename\", engine='pyarrow').

### Data Files

#### File: LacinHLTable_-_HL_table.csv

**Description:** Hemilineage classification table

##### Variables

* Neuroblast: Neuroblast identity
* Notch: Notch on or off
* HL: hemilineage
* NT: neurotransmitter
* Marker_1: genetic marker 1
* Marker_2: genetic marker 2
* Marker_3: genetic marker 3

#### Files: 52A01DBD_GtACR1.pq, R48A07_R20C06_GtACR1.pq, R48A07_R20C06_ChrimsonR.pq, and 52A01DBD_ChrimsonR.pq

**Description:** Optogenetic silencing (GtACR1) and activation (ChrimsonR) of CxHP8 and genetic control datasets.

##### Joint Angles

There are three ty...,