Fig4G_RACE_STOML2_R2_LG350.sgd

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1)  or vector, and luciferase reporters with a 15 bp inserted sequences from the STOMl2, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contanins mutations in GC basepairs that preserve the hairpin structure. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.