Effect of existence or depletion of LIN28B on gene expression during tumor growth of PDAC cells. Effect of existence or depletion of LIN28B on gene expression during tumor growth of PDAC cells

To investigate the effect of existence or depletion of LIN28B in PDAC growth , we established cell lines with or without LIN28B expression. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different cells.Additionally, we performed the whole exome sequencing of 14837T and 15376T to compare their differences Overall design: 9 samples. RNA of 15376T, LIN28B KO 15376T and 14837T were harvested using Trizol reagent. 3 ug of total RNA is used for the construction of sequencing libraries by Hiseq 4000 PE150. Additionally, 3 samples. Genomic DNA of 15376T, 14837T and WT tail tissue were harvested. 1 ug of genomic DNA is used for the construction of sequencing libraries